Renu B ) Studies on Sporulation in Bacillus Anthracis
نویسندگان
چکیده
Concludes that conditions during sporulation play significant role in Bacillus anthracis sporulation. Various factors namely temperature, pH and nutrient deprivation were found to bring about changes in sporulation efficiencies of B. anthracis cultures. B. anthracis spores formed under various stress conditions have varied suceptibility towards physical and chemical denaturants used for spore inactivation. Spores formed at 45 degree Celcius were observed to be more resistant while those formed in nutrient deficient medium (PBS) were more sensitive as compared to spores formed at 37 degree celcius in mG sporulation medium (control). Spores of B. anthracis formed under various stress conditions vary in their age of germination and dipicolinic acid content (DPA). Amount of DPA was observed to be two fold higher in spores formed at 45 degree celcius as compared to spores formed at 37 degree celcius. Atomic force microscopy revealed significant variation in size of spores of B. anthracis formed at different temperatures during sporulation. Spores formed at 45 degree celcius were smaller whereas spores formed at 25 degree celcius were elongated as compared to spores formed at 37 degree celcius. Analysis of proteins of spores formed in PBS and in mG sporulation medium revealed the downregulation of metabolic proteins and upregulation of stress proteins specifically those involved in oxidative stress.
منابع مشابه
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